This is the tutorial of this shiny app. The pipeline is used to compute GSEA analysis on RNA-seq data with two-group experimental design. For example, supposed that you have 3 replicates in group 1 and group 2, respectively, you want to compare the gene expression between these two groups.

How to initiate the app?

  1. Please double click the GSEA_20240318.R file to access the shiny app in Rstudio.
  2. If necessary, please click “install” (1) to install the packages. And click “Run App” to initiate the app (2).
  3. Input “a” to update all the packages if necessary.
  4. Open the app in the browser (3) for better user experience.




What are the inputs?

There are two required inputs for the analysis: read count matrix and metadata. Feel free to access the examples in the /example directory.