Activity Role Overview
In this activity, I visited Kaohsiung Chang Gung Memorial Hospital on October 2, 2024, to introduce our company’s RNA-seq bioinformatics pipeline and help the audience understand how RNA-seq data are processed, analyzed, and interpreted in a service-oriented workflow. My role was to explain the full analysis process, from read quality control, trimming, reference genome alignment, gene expression quantification, TPM/FPKM normalization, and differential expression analysis, to downstream interpretation using GO/KEGG enrichment analysis, ORA, GSEA, pathway visualization, and PPI network analysis. The goal of the session was to make RNA-seq analysis results easier to understand for clinicians and researchers, especially how common output figures such as MultiQC summaries, MA plots, volcano plots, PCA plots, heatmaps, enrichment dot plots, enrichment maps, GSEA plots, Pathview figures, and PPI networks can be interpreted and connected back to biological questions.
Slides
高長庚_台基盟RNAseq_林俊廷_20241002.pptx — part 1 of 3
高長庚_台基盟RNAseq_林俊廷_20241002.pptx — part 2 of 3
高長庚_台基盟RNAseq_林俊廷_20241002.pptx — part 3 of 3
Download all parts to one directory and combine them: cat f3c508f0821bcb542d28b7783958cb0070072ea3d258988c552080e8868632e2.pptx.part01 f3c508f0821bcb542d28b7783958cb0070072ea3d258988c552080e8868632e2.pptx.part02 f3c508f0821bcb542d28b7783958cb0070072ea3d258988c552080e8868632e2.pptx.part03 > f3c508f0821bcb542d28b7783958cb0070072ea3d258988c552080e8868632e2.pptx.
Verify with shasum -a 256 f3c508f0821bcb542d28b7783958cb0070072ea3d258988c552080e8868632e2.pptx. Expected SHA-256: f3c508f0821bcb542d28b7783958cb0070072ea3d258988c552080e8868632e2.
Download ## Slides (PPTX) — part 1 of 3 Download ## Slides (PPTX) — part 2 of 3 Download ## Slides (PPTX) — part 3 of 3